Blar i forfatter "Vanderpoorten, Oliver"

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    • OptiJ: Open-source optical projection tomography of large organ samples 

      Vallejo Ramirez, Pedro P.; Zammit, Joseph; Vanderpoorten, Oliver; Fergus, Riche; Blé, Francois-Xavier; Zhou, Xiao-Hong; Spiridon, Bogdan; Valentine, Christopher; Spasov, Simeon E.; Oluwasanya, Pelumi W.; Goodfellow, Gemma; Fantham, Marcus J.; Siddiqui, Omid; Alimagham, Farah; Robbins, Miranda; Stretton, Andrew; Simatos, Dimitrios; Hadeler, Oliver; Rees, Eric J.; Ströhl, Florian; Laine, Romain F.; Kaminski, Clemens F. (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-10-30)
      The three-dimensional imaging of mesoscopic samples with Optical Projection Tomography (OPT) has become a powerful tool for biomedical phenotyping studies. OPT uses visible light to visualize the 3D morphology of large transparent samples. To enable a wider application of OPT, we present OptiJ, a low-cost, fully open-source OPT system capable of imaging large transparent specimens up to 13 mm tall ...

    • Rapid prototyping of 1xN multifocus gratings via additive direct laser writing 

      Reischke, Marie; Vanderpoorten, Oliver; Ströhl, Florian (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-04-05)
      Multifocus gratings (MFGs) enable microscopes and other imaging systems to record entire Z-stacks of images in a single camera exposure. The exact grating shape depends on microscope parameters like wavelength and magnification and defines the multiplexing onto a grid of MxN Z-slices. To facilitate the swift production and alteration of MFGs for a system and application at hand, we have developed ...

    • Single-Molecule Sizing through Nanocavity Confinement 

      Jacquat, Raphaël P. B.; Krainer, Georg; Peter, Quentin A. E.; Babar, Ali Nawaz; Vanderpoorten, Oliver; Xu, Catherine K.; Welsh, Timothy J.; Kaminski, Clemens F.; Keyser, Ulrich F.; Baumberg, Jeremy J.; Knowles, Tuomas P. J. (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-02-24)
      : An approach relying on nanocavity confinement is developed in this paper for the sizing of nanoscale particles and single biomolecules in solution. The approach, termed nanocavity diffusional sizing (NDS), measures particle residence times within nanofluidic cavities to determine their hydrodynamic radii. Using theoretical modeling and simulations, we show that the residence time of particles ...

    • Superresolving the kidney – a practical comparison of fluorescence nanoscopy of the glomerular filtration barrier 

      Wunderlich, Lucia; Ströhl, Florian; Ströhl, Stefan; Vanderpoorten, Oliver; Mascheroni, Luca; Kaminski, Clemens F. (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-12-05)
      Immunofluorescence microscopy is routinely used in the diagnosis of and research on renal impairments. However, this highly specific technique is restricted in its maximum resolution to about 250 nm in the lateral and 700 nm in the axial directions and thus not sufficient to investigate the fine subcellular structure of the kidney’s glomerular filtration barrier. In contrast, electron microscopy ...

    • A waveguide imaging platform for live-cell TIRF imaging of neurons over large fields of view 

      Opstad, Ida Sundvor; Ströhl, Florian; Fantham, Marcus J.; Hockings, Colin; Vanderpoorten, Oliver; Tartwijk, Francesca W. van; Qiaojin Lin, Julie; Tinguely, Jean-Claude; Dullo, Firehun Tsige; Kaminski‐Schierle, Gabriele S.; Ahluwalia, Balpreet Singh; Kaminski, Clemens F. (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-02-17)
      Large fields of view (FOVs) in total internal reflection fluorescence microscopy (TIRFM) via waveguides have been shown to be highly beneficial for single molecule localisation microscopy on fixed cells [1,2] and have also been demonstrated for short‐term live‐imaging of robust cell types [3‐5], but not yet for delicate primary neurons nor over extended periods of time. Here, we present a waveguide‐based ...

    • α-Synuclein fibril and synaptic vesicle interactions lead to vesicle destruction and increased lipid-associated fibril uptake into iPSC-derived neurons 

      Stephens, Amberley D.; Villegas, Ana Fernandez; Chung, Chyi Wei; Vanderpoorten, Oliver; Pinotsi, Dorothea; Mela, Ioanna; Ward, Edward; McCoy, Thomas M.; Cubitt, Robert; Routh, Alexander F.; Kaminski, Clemens F.; Kaminski Schierle, Gabriele S. (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-05-15)
      Monomeric alpha-synuclein (aSyn) is a well characterised protein that importantly binds to lipids. aSyn monomers assemble into amyloid fibrils which are localised to lipids and organelles in insoluble structures found in Parkinson’s disease patient’s brains. Previous work to address pathological aSyn-lipid interactions has focused on using synthetic lipid membranes, which lack the complexity of ...